A label-free double-amplification system for sensitive detection of single-stranded DNA and thrombin by liquid chromatography-mass spectrometry.
نویسندگان
چکیده
A label-free double amplification system has been developed by using a ternary DNA probe containing the poly(adenine-thymine) sequence assisted by exonuclease III degradation. The method achieved more than 600-fold signal amplification and allowed sensitive detection of single-stranded DNA and thrombin at the pM level by using liquid chromatography/mass spectrometry.
منابع مشابه
Supporting Information A label-free double-amplification system for sensitive detection of single-stranded DNA and thrombin by liquid chromatography-mass spectrometry
Chemicals All the oligonucleotides were purchased from Beijing AuGCT DNA-SYN Biotechnology Co. Ltd (Beijing, China). Exonuclease III (Exo III) along with the reaction buffer, 10 × NEBuffer 1 (100 mM bis-tris-propane-HCl, 100 mM MgCl 2 and 10.0 mM dithiothreitol, pH 7.0) and 10 × NEBuffer 3 (100 mM NaCl, 50 mM Tris-HCl, 10 mM MgCl 2 , 1 mM dithiothreitol pH 7.9) was purchased from New England Bi...
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ورودعنوان ژورنال:
- Chemical communications
دوره 50 69 شماره
صفحات -
تاریخ انتشار 2014